Analysis data

Required input

• Analysis: a description of the analysis, e.g. native semen, after preparation, etc.
• Technician: the login name of the technician who performs or has performed the semen analysis.
• Room temperature: enter the room temperature (optional).
• Preparation depth: this is the depth of the counting chamber derived from the known volume of semen and the dimensions (length and width) of the cover-glass:
  

  

  The default sample preparation depth is entered in the Options section of the program.
• Dilution: if the semen used for analysis is diluted then you must enter the proportion of semen and the proportion of diluent. If the semen is not diluted, enter the value 1 for proportion of semen and the value 0 for proportion of diluent. The default dilution is entered in the Options section of the program.
• Time after ejaculation: the time interval between time of ejaculation and the time of analysis, i.e. the current time, is calculated by the program. If the value is not displayed or is not correct, you can enter the correct time interval between ejaculation and semen analysis.

Analysis

Click the Start analysis button to start the counting procedure.

Results

• The number of cells analyzed;
• The number of motile cells counted;
• Sperm concentration in 10⁶/mL;
• Proportion of spermatozoa with grade (a), (b), (c) and (d) motility (in %) and concentration of spermatozoa with grade (a), (b), (c) and (d) motility (in 10⁶/mL).

  A spermatozoon is classified as:

  • grade (a): if it has a rapid linear progressive motility with linear velocity in excess of the lower limit selected in the Options box.
  • grade (b): if it has a slow or sluggish linear or non-linear movement; such a spermatozoon has a linear velocity below the higher limit selected in the Options box, but a velocity in excess of the lower limit selected in the in the Options box;
  • grade (c): if it has a non-progressive motility; its velocity is below the higher limit selected in the Options box;
  • grade (d): if the spermatozoon is immotile.
• Mean, standard deviation and median of velocity, linear velocity and linearity index, of grade (a), (b) and (c) motile cells only.
  • Velocity is the total distance covered by the spermatozoa per unit of time and is expressed in μm/sec.
    
  • Linear Velocity is the shortest distance between the first and the last point of measurement, calculated per unit of time and is expressed in μm/sec (note that this is the theoretical definition; AutoSperm uses a more advanced method for estimating linearity of cell movement).
  • Linearity Index (%) is the quotient of linear velocity divided by velocity, multiplied by 100.

Graphs

• Click on Velocity graph, Linear Velocity or Linearity Index to obtain a histogram for the corresponding parameter.

  

Buttons

• Print: print a complete report
• OK: saves the data in the database and closes the dialog box
• Cancel: closes the dialog box without saving the data in the database.

Tips

• When you click Print, the report will immediately be printed to the default printer. If you want to select a printer first, press Ctrl+P or function key F11.
• Remember that when you switch from one tab to another, the changes that you have made are saved in the database. If you do not want your recent changes to be saved, click the Cancel button.